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和铂医药(上海)有限责任公司

和铂医药(上海)有限责任公司

所属行业: 显示/光电显示  
公司地址:上海市浦东新区哈雷路866号311室(邮编:201203) 公司官网:http://www.harbourbiomed.com

公司简介

招聘职位

Phage display Scientist

Phage display Scientist

10000-20000 上海-浦东新区 经验不限 不限学历 全职 招聘 若干 人 年龄不限 更新时间:2018-08-15

投递简历

职责描述

Perform total RNA extraction and RT-PCR:Mouse single B cell suspension preparation;Extract high quality total RNA by Trizol reagent;Conduct first strand cDNA synthesis.Perform antibody phage libraries construction:Conduct the first round PCR and gel purification of 1st PCR product and sequence quality analysis;Conduct and optimize the second overlap PCR and sequence quality analysis;Prepare high transformation efficiency electro-competent cells;Be responsible for phage library packing and purification.Perform antibody phage libraries panning and screening:Perform QC of antigen/biotinylated antigen;Conduct phage solid, liquid and cell panning;Conduct colony picking and culture and induction;Perform high-throughput ELSIA or mirrorball screening;Re-array positive isolates and sequencing;Perform unique sequence analysis and characterization.Coordinate lead isolation characterization:Closely work with biology team and support antibody discovery;Keep appropriate experimental records and documentation: A. Education/training background:Master with 2+ years, or BS with 5+ years of experience Molecular Biology, Biochemistry, cell biology etc.; At least 2 years working experience in pharmaceutical or biotech companies is required. B. Knowledge/Skills:Mastery of molecular biology(e.g. PCR, RT-PCR, molecular cloning etc.).Hands-on experience in phage display technology.Knowledge of antibody properties, structure and sequences is required. Knowledge of molecular biology or protein chemistry or structure biology is plus. Experience of therapeutic antibody is plus.Excellent data management is plus.Excellent presentation skills is plus.Experience of antibody repertoire analysis is plus.Experience of protein structure modeling is plus.Experience of following software / tools is plus:

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